Resistance is futile: evaluating and modifying cellular pathways during AAV vector expression in HEK293 cells to increase viral titers

被引：0
|
作者：
Kraemer, N. [1 ,3 ]
Teschner, K. [2 ]
Ortseifen, V. [1 ]
Sundermann, F. [1 ]
Droste, J. [2 ]
Northoff, S. [1 ]
Klausing, S. [1 ]
机构：
[1] Sartorius Xell GmbH, D-33689 Bielefeld, Germany
[2] Sartorius Xell GmbH, D-33758 Schloss Holte Stukenbroc, Germany
[3] Univ Bielefeld, Fac Technol, Cell Culture Technol, D-33615 Bielefeld, Germany
来源：
HUMAN GENE THERAPY | 2022年 / 33卷 / 23-24期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
P484
引用
收藏
页码：A153 / A154
页数：2
相关论文
共 32 条
  • [1] Cellular signaling pathways modulated by influenza infection in HEK293 cells
    Aziza P Manceur
    Amine A Kamen
    Emma Petiot
    Chun F Shen
    Sven Ansorge
    [J]. BMC Proceedings, 9 (Suppl 9)
  • [2] AAV Packaging in HEK293 Cells Prefers Unit-Length Vector Genomes
    Li, Xin
    Miller, Lohra Mickelle
    Frabutt, Dylan A.
    Lam, Anh K.
    Draper, Benjamin E.
    Jarrold, Martin F.
    Herzog, Roland W.
    Xiao, Weidong
    Zhang, Junping
    [J]. MOLECULAR THERAPY, 2023, 31 (04) : 506 - 507
  • [3] PLATFORM FOR THE GROWTH AND PROPOGATION OF HEK293 CELLS AND ADENOVIRUS VIRAL VECTOR AMPLIFICATION
    Sanderson, T.
    Joseph, T.
    Haq, T.
    Lefebvre, P.
    Gantier, R.
    [J]. CYTOTHERAPY, 2019, 21 (05) : S24 - S25
  • [4] CRISPR Screening Identifies Gene Knockouts That Improve Lentiviral Vector Titers in HEK293 Cells
    Iaffaldano, Brian
    Marino, Michael
    Reiser, Jakob
    [J]. MOLECULAR THERAPY, 2020, 28 (04) : 422 - 422
  • [5] In vitro and in vivo comparability assessment of an AAV vector manufactured by triple transfection in HEK293 cells or in the baculovirus expression system
    Steel, M.
    Woodburn, K.
    Kniffin, T.
    Gebretsadik, K.
    Chan, J.
    Vijay, S.
    Chen, H.
    Chalberg, T. W.
    Gasmi, M.
    [J]. HUMAN GENE THERAPY, 2015, 26 (10) : A70 - A70
  • [6] Reconfiguration of AAV Rep-Cap coding sequences significantly increases viral vector yield and enables inducible AAV production in HEK293 cells
    Bray, A.
    Janer, A. Barber
    Branciaroli, C.
    Su, W.
    Liu, Q.
    Payne, T.
    Cawood, R.
    [J]. HUMAN GENE THERAPY, 2018, 29 (12) : A154 - A154
  • [7] Study on vector elements for efficient selection, expression and secretion in HEK293 cells
    Roman, Ramon
    Miret, Joan
    Martinez, Ivan
    Roura, Aida
    Casablancas, Antoni
    Lecina, Marti
    Cairo, Jordi J.
    [J]. NEW BIOTECHNOLOGY, 2016, 33 : S189 - S189
  • [8] Adenovirus Vector With ADP Gene Induces Cytopathic Effects in HEK293 Cells Without Significant Elevation of Virus Titers
    Shiota, Aoi
    Nishimae, Fumitaka
    Sakurai, Fuminori
    Mizuguchi, Hiroyuki
    [J]. ANTICANCER RESEARCH, 2022, 42 (04) : 1719 - 1727
  • [9] Characterization of residual microRNAs in AAV vector batches produced in HEK293 mammalian cells and Sf9 insect cells
    Penaud-Budloo, Magalie
    Lecomte, Emilie
    Lecomte, Quentin
    Pacouret, Simon
    Broucque, Frederic
    Guy-Duche, Aurelien
    Dupont, Jean-Baptiste
    Jeanson-Leh, Laurence
    Robin, Cecile
    Blouin, Veronique
    Ayuso, Eduard
    Adjali, Oumeya
    [J]. MOLECULAR THERAPY METHODS & CLINICAL DEVELOPMENT, 2024, 32 (03)
  • [10] Vector Engineering of pRep-Cap and pHelper Enhanced AAV Productivity by Triple Transfection in Suspension HEK293 Cells
    Tripp, Caitlin
    Wang, Jennifer
    Chen, Tsai-Yu
    Dong, Wenling
    Ramaswamy, Senthil
    Gu, Bingnan
    [J]. MOLECULAR THERAPY, 2021, 29 (04) : 62 - 62
1234