Dextran and fructose separation on an SMB continuous chromatographic unit

A dextran/fructose mixture was obtained by fermentation of a sucrose rich media using Leuconostoc mesenteroides NRRL B512(f). The dextran and fructose mixture obtained by fermentation as well as a synthetic mixture of pure components were separated using simulated moving bed (SMB) chromatography. In the case of the synthetic feed, the purity in the raffinate (dextran) and extract (fructose) was 96 and 81%, respectively. For the fermented mixture the purity values were 87.2% for the extract and 100% for the raffinate. (C) 2002 Elsevier Science B.V. All rights reserved.