Expression, Purification and Structural Characterization of Up-Regulated Gene 7 Encoded Protein

被引:3
|
作者
Ostuni, Angela [1 ]
Morelli, Maria Antonietta Castiglione [1 ]
Miglionico, Rocchina [1 ]
Salvia, Antonella Maria [1 ]
Cuviello, Flavia [1 ]
Bisaccia, Faustino [1 ]
机构
[1] Univ Basilicata, Dept Sci, I-85100 Potenza, Italy
来源
PROTEIN AND PEPTIDE LETTERS | 2014年 / 21卷 / 05期
关键词
Circular dichroism; fluorescence; hepatocellular carcinogenesis; NMR; structure; URG7; X ANTIGEN EFFECTOR; SECONDARY-STRUCTURE; PEPTIDES; URG7;
D O I
10.2174/092986652105140218105249
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Up-Regulated Gene 7 (URG7) is a host gene up-regulated in HBV infected hepatocytes that has been suggested to have an anti-apoptotic activity mediated by caspases 3 and 8 and an endoplasmic reticulum localization. Here we report the structural characterization of the encoded protein URG7 by circular dichroism and fluorescence spectroscopy in different solvent media: phosphate buffer and two membrane-mimetic solvents, i.e. 2,2,2-trifluoroethanol (TFE) and SDS micelles. In all solvents URG7 contains substantial amounts of secondary structures. To obtain information about the structural organization and stability of URG7, its thermal denaturation in a membrane environment was studied and intermediate states of thermal unfolding were observed. Furthermore, fluorescence results in SDS micelles could be compatible with different environments for the four tryptophan residues in URG7. Preliminary NMR data indicate that URG7 in TFE solution is quite flexible and not well folded. These data are the first structural information on URG7 and might provide an insight into its structure-function relationships.
引用
收藏
页码:413 / 418
页数:6
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