Comparable constitutive expression and activity of cytochrome P450 between the lobes of the porcine liver

被引：4

作者：

Rasmussen, Martin Kroyer ^{[1
]}

Zamaratskaia, Galia ^{[2
]}

Ekstrand, Bo ^{[1
]}

机构：

[1] Aarhus Univ, Dept Food Sci, DK-8830 Tjele, Denmark

[2] Swedish Univ Agr Sci, Dept Food Sci, Uppsala, Sweden

来源：

TOXICOLOGY IN VITRO | 2014年 / 28卷 / 07期

关键词：

Porcine liver; Model system; Detoxification; PCA; Inter-individual; Intra-hepatic; HEPATIC MICROSOMES; GENE-EXPRESSION; PIG-LIVER;

D O I：

10.1016/j.tiv.2014.06.002

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Due to limited availability of human liver tissue for the study of cytochrome P450 (CYP450), porcine liver tissue has been suggested as an alternative source to prepare microsomes and hepatocytes. The porcine liver is made by four different lobes. The present study investigated the expression and activity of specific CYP450 isoforms in the four lobes, with the purpose to examine if one lobe of the porcine liver resembles the human more than others. Samples from the four major lobes were taken from female pigs and mRNA expression and activity of CYP1A, 2A, 2C, 2D, 2E and 3A determined. The results showed no differences in specific mRNA expression and activity of any of the investigated CYP450 isoforms. In conclusion, the study shows that all parts of the porcine liver are equally useful as model tissue. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1190 / 1195

页数：6

共 50 条

[1] Constitutive expression and activity of cytochrome P450 in conventional pigs
Nielsen, Soren Drud
Bauhaus, Yvonne
Zamaratskaia, Galia
Junqueira, Matheus Antunes
Blaabjerg, Karoline
Petrat-Melin, Bjorn
Young, Jette Feveile
Rasmussen, Martin Kroyer
RESEARCH IN VETERINARY SCIENCE, 2017, 111 : 75 - 80
[2] Expression and Induction of Cytochrome P450 Activity in Cryopreserved Porcine Hepatocytes.
Cursio, Raffaele
Baldini, Edoardo
Margara, Andrea
Honiger, Jiri
Saint-Paul, Marie-Christine
Bayer, Pascal
Raymondi, Vincent
Rahmani, Roger
Mouiel, Jean
Gugenheim, Jean
LIVER TRANSPLANTATION, 2012, 18 : S147 - S148
[3] Correlation of Cytochrome P450 Oxidoreductase Expression with the Expression of 10 Isoforms of Cytochrome P450 in Human Liver
Zhang, Hai-Feng
Li, Zhi-Hui
Liu, Jia-Yu
Liu, Ting-Ting
Wang, Ping
Fang, Yan
Zhou, Jun
Cui, Ming-Zhu
Gao, Na
Tian, Xin
Gao, Jie
Wen, Qiang
Jia, Lin-Jing
Qiao, Hai-Ling
DRUG METABOLISM AND DISPOSITION, 2016, 44 (08) : 1193 - 1200
[4] The effect of the glutamate system on liver cytochrome P450 expression and activity
Bromek, E.
Haduch, A.
Rysz, M.
Jastrzebska, J.
Puklo, R.
Daniel, W. A.
EUROPEAN NEUROPSYCHOPHARMACOLOGY, 2021, 53 : S665 - S665
[5] Cytochromes P450 .6. Constitutive expression of hepatic cytochrome P450 genes
Gonzalez, FJ
Lee, YH
FASEB JOURNAL, 1996, 10 (10): : 1112 - 1117
[6] Porcine cytochrome P450 and metabolism
Skaanild, Mette Tingleff
CURRENT PHARMACEUTICAL DESIGN, 2006, 12 (11) : 1421 - 1427
[7] Comparison of human and porcine cytochrome P450 expression.
Liu, J
Yano, N
Rifai, A
Naik, S
Santangini, H
Trenkler, D
Jauregui, HO
HEPATOLOGY, 1998, 28 (04) : 493A - 493A
[8] Constitutive expression of cytochrome P450 in foetal and adult porcine livers-Effects of body weight
Rasmussen, Martin Kroyer
Theil, Peter Kappel
Oksbjerg, Niels
TOXICOLOGY LETTERS, 2016, 258 : 87 - 92
[9] In vitro effects of rebaudioside A, stevioside and steviol on porcine cytochrome p450 expression and activity
Thogersen, Rebekka
Petrat-Melin, Bjorn
Zamaratskaia, Galia
Grevsen, Kai
Young, Jette Feveile
Rasmussen, Martin Kroyer
FOOD CHEMISTRY, 2018, 258 : 245 - 253
[10] Cytochrome P450 expression and catalytic activity in coronary arteries and liver of cattle
Grasso, E
Longo, V
Coceani, F
Gervasi, PG
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2005, 1722 (02): : 116 - 123

← 1 2 3 4 5 →