YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase

被引：31
|
作者：
Kol, Stefan
Turrell, Bryony R.
de Keyzer, Jeanine
van der Laan, Martin
Nouwen, Nico
Driessen, Arnold J. M. [1 ]
机构：
[1] Univ Groningen, Dept Mol Microbiol, Groningen Biomol Sci & Biotechnol Inst, NL-9751 NN Haren, Netherlands
[2] Univ Groningen, Mat Sci Ctr Plus, NL-9751 NN Haren, Netherlands
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2006年 / 281卷 / 40期
关键词：
D O I：
10.1074/jbc.M605317200
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F1F0 ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F1F0 ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC- dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.
引用
收藏
页码：29762 / 29768
页数：7
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