Results of an International Interlaboratory Trial to Determine Twelve Allergens Using Real-time PCR- and ELISA-based Assays

被引：1
|
作者：
Koeppel, Rene [1 ]
Rentsch, Juerg [2 ]
Ruf, Juerg [3 ]
Eugster, Albert [4 ]
Graf, Christoph [5 ]
Felderer, Nora [1 ]
Pietsch, Klaus [6 ]
Ilg, Evelyn [7 ]
机构：
[1] Official Food Control Author Canton Zurich, Zurich, Switzerland
[2] Swiss Qual Testing Serv SQTS Courtepin, Zurich, Switzerland
[3] Official Food Control Author Canton Thurgau, Frauenfeld, Switzerland
[4] Cantonal Off Consumer Protect Aargau, Aarau, Switzerland
[5] Cantonal Off Consumer Protect Bern, Bern, Switzerland
[6] State Inst Chem & Veterinarian Anal, Freiburg, Germany
[7] Cantonal Food Control Author Basel, Basel, Switzerland
来源：
CHIMIA | 2014年 / 68卷 / 10期
关键词：
Allergen; Determination; ELISA; Real-time PCR; Sausages; CHAIN-REACTION PCR; FOOD;
D O I：
10.2533/chimia.2014.721
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
To elucidate the capability of laboratories to determine allergen contents, an international interlaboratory trial was conducted using meat products spiked with 12 allergens. The measurement uncertainty was calculated independent of the applied method simulating realistic situations when comparing analysis certificates from different laboratories. The measurement uncertainty was revealed to be in the best cases +/-100%, in the worst cases quantification exhibited a measurement uncertainty of higher than 200% making quantitative analysis impossible. The measurement uncertainty seemed to depend on the analyte and assays used.
引用
收藏
页码：721 / 725
页数：5
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