Oxidative stress is a likely molecular mechanism in the neurotoxicity of kainic acid (KA), an excitotoxic substance. The aim of this report was to assess the effect qf melatonin co-treatment against KA by measuring the levels of Coenzyme Q10 (CoQ 10), lipid peroxidation (LPO), and Thioredoxin (Trx) mRNA in the rat hippocampus. The male rats were divided into three groups as saline, KA treatment (15 mg/kg), and KA plus melatonin (20 mg/kg). The levels of LPO and CoQ10 were determined by high pressure liquid chromatography (HPLC) consisting of fluorescence and electro-chemical detectors, respectively. The expression of the Trx gene was quantified using reverse transcription followed by real-time polymerase chain reaction (RT-PCR). The results show that the level of LPO increased although the level of CoQ 10 decreased both in homogenates and mitochondria in KA-treated rats However, melatonin co-treatment attenuated the level of LPO and partially restored the level of CoQ10. Melatonin co-treatment against KA did not affect the regulation of Trx. Finally, in the context of the decreased LPO and the increased CoQ10, the results suggest that melatonin may be protective against central nervous system pathologies involving excitotoxicity or where oxidative damage may contribute to mitochondrial dysfunction.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Nogueira, Bruno G.
Pereira, Raiza R.
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Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Pereira, Raiza R.
Bitencourt, Julia L.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Bitencourt, Julia L.
Milan, Bruno
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Milan, Bruno
Reis, Willian V. A.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Reis, Willian V. A.
Mozarth Junior, V.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Mozarth Junior, V.
Acacio, Bianca R.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Acacio, Bianca R.
Sampaio, Breno F. B.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Sampaio, Breno F. B.
Souza, Maria I. L.
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Fed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
Fed 24 Univ Mato Grosso Do Sul, Fac Vet Med & Zootech, FAMEZ, Ave Senador Filinto 26 Muller,2443-Univ City,Mailb, Campo Grande BR-790702790, MS, BrazilFed Univ Mato Grosso Fdn, Fac Vet Med & Anim Sci, FAMEZ, UFMS, Campo Grande, MS, Brazil
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Adnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, TurkeyAdnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, Turkey
Gules, Ozay
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Kum, Sadiye
Yildiz, Mustafa
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Canakkale Onsekiz Mart Univ, Can Sch Appl Sci, Dept Occupat Hlth & Safety, Canakkale, TurkeyAdnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, Turkey
Yildiz, Mustafa
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Boyacioglu, Murat
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Ahmad, Ejaz
Naseer, Zahid
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Pir Mehr Ali Shah Arid Agr Univ, Fac Vet & Anim Sci, Dept Clin Studies, Rawalpindi, PakistanAdnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, Turkey
Naseer, Zahid
Eren, Ulker
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Adnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, TurkeyAdnan Menderes Univ, Fac Vet Med, Dept Histol & Embryol, Aydin, Turkey