Effect of melatonin cotreatment against kainic acid on coenzyme Q10 lipid peroxidation and Trx mRNA in rat hippocampus

被引:16
|
作者
Yalcin, A [1 ]
Kilinc, E
Kocturk, S
Resmi, H
Sozmen, EY
机构
[1] Ege Univ, Fac Pharm, Dept Biochem, TR-35100 Izmir, Turkey
[2] Ege Univ, Fac Pharm, Dept Analyt Chem, Izmir, Turkey
[3] Dokuz Eylul Univ, Fac Med, Dept Biochem, Izmir, Turkey
[4] Ege Univ, Fac Med, Dept Biochem, Izmir, Turkey
关键词
coenzyme Q10; kainic acid; lipid peroxidation; melatonin; thioredoxin;
D O I
10.1080/00207450490475535
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Oxidative stress is a likely molecular mechanism in the neurotoxicity of kainic acid (KA), an excitotoxic substance. The aim of this report was to assess the effect qf melatonin co-treatment against KA by measuring the levels of Coenzyme Q10 (CoQ 10), lipid peroxidation (LPO), and Thioredoxin (Trx) mRNA in the rat hippocampus. The male rats were divided into three groups as saline, KA treatment (15 mg/kg), and KA plus melatonin (20 mg/kg). The levels of LPO and CoQ10 were determined by high pressure liquid chromatography (HPLC) consisting of fluorescence and electro-chemical detectors, respectively. The expression of the Trx gene was quantified using reverse transcription followed by real-time polymerase chain reaction (RT-PCR). The results show that the level of LPO increased although the level of CoQ 10 decreased both in homogenates and mitochondria in KA-treated rats However, melatonin co-treatment attenuated the level of LPO and partially restored the level of CoQ10. Melatonin co-treatment against KA did not affect the regulation of Trx. Finally, in the context of the decreased LPO and the increased CoQ10, the results suggest that melatonin may be protective against central nervous system pathologies involving excitotoxicity or where oxidative damage may contribute to mitochondrial dysfunction.
引用
收藏
页码:1085 / 1097
页数:13
相关论文
共 50 条
  • [1] Effect of melatonin on the regulation of proenkephalin and prodynorphin mRNA levels induced by kainic acid in the rat hippocampus
    Won, JS
    Song, DK
    Huh, SO
    Kim, YH
    Suh, HW
    HIPPOCAMPUS, 2000, 10 (03) : 236 - 243
  • [2] Coenzyme Q10 and α-Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen
    Nogueira, B. G.
    Sampaio, B. F. B.
    Souza, M. I. L.
    Costa e Silva, E. V.
    Zuccari, C. E. S. N.
    REPRODUCTION IN DOMESTIC ANIMALS, 2015, 50 (06) : 1003 - 1010
  • [3] Coenzyme Q10 and melatonin protect cryopreserved equine sperm against lipoperoxidation
    Nogueira, Bruno G.
    Pereira, Raiza R.
    Bitencourt, Julia L.
    Milan, Bruno
    Reis, Willian V. A.
    Mozarth Junior, V.
    Acacio, Bianca R.
    Sampaio, Breno F. B.
    Souza, Maria I. L.
    ANIMAL REPRODUCTION SCIENCE, 2022, 243
  • [4] Augmenting effect of vitrification on lipid peroxidation in mouse preantral follicle during cultivation: Modulation by coenzyme Q10
    Kashka, Roya Hedayati
    Zavareh, Saeed
    Lashkarbolouki, Taghi
    SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, 2016, 62 (06) : 404 - 414
  • [5] Preventive effect of melatonin against brain mitochondria DNA damage, lipid peroxidation and seizures induced by kainic acid
    Mohanan, PV
    Yamamoto, H
    TOXICOLOGY LETTERS, 2002, 129 (1-2) : 99 - 105
  • [6] Protective role of melatonin and coenzyme Q10 in ochratoxin a toxicity in rat liver and kidney
    Sutken, Emine
    Aral, Erinc
    Ozdemir, Filiz
    Uslu, Sema
    Alatas, Ozkan
    Colak, Omer
    INTERNATIONAL JOURNAL OF TOXICOLOGY, 2007, 26 (01) : 81 - 87
  • [7] Investigation of protective effect of resveratrol and coenzyme Q10 against cyclophosphamide-induced lipid peroxidation, oxidative stress and DNA damage in rats
    Akbel, Erten
    Kucukkurt, Ismail
    Ince, Sinan
    Demirel, Hasan Huseyin
    Acaroz, Damla Arslan
    Zemheri-Navruz, Fahriye
    Kan, Fahriye
    TOXICOLOGY RESEARCH, 2024, 13 (01)
  • [8] THE EFFECTS OF COENZYME Q10 AND BAICALIN IN CISPLATIN-INDUCED LIPID PEROXIDATION AND NITROSATIVE STRESS
    Sawicka, Ewa
    Dlugosz, Anna
    Rembacz, Krzysztof P.
    Guzik, Anna
    ACTA POLONIAE PHARMACEUTICA, 2013, 70 (06): : 977 - 985
  • [9] Protective effect of coenzyme Q10 against bisphenol-A-induced toxicity in the rat testes
    Gules, Ozay
    Kum, Sadiye
    Yildiz, Mustafa
    Boyacioglu, Murat
    Ahmad, Ejaz
    Naseer, Zahid
    Eren, Ulker
    TOXICOLOGY AND INDUSTRIAL HEALTH, 2019, 35 (07) : 466 - 481
  • [10] Protective Effect of Coenzyme Q10 Against I131 Toxicity in Rat Livers
    Akbulut, A.
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2018, 45 : S625 - S625