Purification, Visualization, and Molecular Signature of Neural Stem Cells

被引：9

作者：

Yu, Yuan Hong ^{[1
]}

Narayanan, Gunaseelan ^{[1
]}

Sankaran, Shvetha ^{[1
]}

Ramasamy, Srinivas ^{[1
]}

Chan, Shi Yu ^{[1
]}

Lin, Shuping ^{[1
]}

Chen, Jinmiao ^{[2
]}

Yang, Henry ^{[2
]}

Srivats, Hariharan ^{[1
]}

Ahmed, Sohail ^{[1
]}

机构：

[1] Inst Med Biol, Neural Stem Cell Lab, Singapore 138648, Singapore

[2] Singapore Immunol Network, Bioinformat Lab, Singapore, Singapore

来源：

STEM CELLS AND DEVELOPMENT | 2016年 / 25卷 / 02期

关键词：

CENTRAL-NERVOUS-SYSTEM; PROGENITOR CELLS; SIGNALING PATHWAY; FLOW-CYTOMETRY; EXPRESSION; POPULATION; IDENTIFICATION; DEFINES; PRECURSORS; SUBCLASSES;

D O I：

10.1089/scd.2015.0190

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1as a novel marker for NSCs and show that it can be used along with Lewis-X (LeX) to yield a highly purified population of NSCs. Using time-lapse microscopy, we are able to follow NSCs forming neurospheres, allowing their visualization. Finally, using single-cell polymerase chain reaction (PCR), we determine the molecular signature of NSCs. The single-cell PCR data suggest that along with the Notch and Shh pathways, the Hippo pathway plays an important role in NSC activity.

引用

页码：189 / 201

页数：13

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