Occurrence of a metalloprotease (thermolysin) inhibitor among Brevibacillus species and purification of such inhibitor from Brevibacillus reuszeri IFO 15719

被引：0

作者：

Kobayashi, T ^{[1
]}

Nakagawa, N ^{[1
]}

Imada, C ^{[1
]}

Hamada-Sato, N ^{[1
]}

Watanabe, E ^{[1
]}

机构：

[1] Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Appl Microbiol Lab, Tokyo 1088477, Japan

来源：

FISHERIES SCIENCE | 2004年 / 70卷 / 02期

关键词：

Brevibacillus laterosporus; Brevibacillus reuszeri; metalloprotease; protease inhibitors; thermolysin;

D O I：

10.1111/j.1444-2906.2003.00804.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The extracellular production of a thermolysin inhibitor by 534 wild-type strains isolated from marine sediments and related-type strains was examined. The inhibition of thermolysin activity by strain T-177 was observed on a casein agar medium. According to our detailed taxonomic study, strain T-177 is related to Brevibacillus laterosporus. Further screening for a thermolysin inhibitor was performed using strain T-177 and nine other taxonomically related Brevibacillus type strains, and the ability to produce the inhibitor was observed in five strains. Among these strains, B. reuszeri IFO 15719(T) exhibited the highest thermolysin inhibitory activity in broth media, and thus was used for the purification and characterization of the inhibitor. Chromatographic analyses suggested that this substance is a monomeric protein with a molecular mass of 60 kDa.

引用

页码：299 / 305

页数：7

共 6 条

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