Analysis of solid-supported oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：0
|
作者：
Meyer, A [1 ]
Spinelli, N [1 ]
Imbach, JL [1 ]
Vasseur, JJ [1 ]
机构：
[1] Univ Montpellier 2, Lab Chim Organ Biomol Synthese, UMR CNRS 5625, F-34095 Montpellier 5, France
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2000年 / 14卷 / 04期
关键词：
D O I：
10.1002/(SICI)1097-0231(20000229)14:4<234::AID-RCM874>3.0.CO;2-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study presents matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as a powerful tool to analyze and characterize oligonucleotides covalently linked to a solid support during their synthesis. The analysis of the fragment ions generated either in negative or positive mode allows direct and easy access to the nucleotide sequence and identification of the internucleosidic linkage. The mechanisms of the fragmentation of the solid-supported oligonucleotides induced by MALDI-TOFMS are discussed. Copyright (C) 2000 John Wiley & Sons, Ltd.
引用
收藏
页码：234 / 242
页数：9
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