Identification of miRNA-1 as a novel regulator of insulin-like growth factor-1 in deer antler proliferation and regeneration

被引：0
|
作者：
Hu, W. [1 ]
Meng, X. Y. [1 ]
Li, T. [1 ]
Li, M. [1 ]
Tian, Y. H. [1 ]
Hu, R. [2 ]
机构：
[1] Jilin Agr Univ, Coll Life Sci, Changchun 130122, Peoples R China
[2] Eastern Liaoning Univ, Coll Agr, Dandong, Liaoning, Peoples R China
来源：
BIOTECHNOLOGY, AGRICULTURE, ENVIRONMENT AND ENERGY | 2015年
关键词：
Deer antler; miRNA-1; Insulin-like growth factor-1; Regeneration; Proliferation; IGF-I; MICRORNAS; VITRO;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In order to investigate miR-1 regulating the expression of IGF-1 in the proliferation of sika deer antler, an miR-1 mimic was transfected into antler cartilage cells. The result showed that miR-1 could bind to the 3'UTR of IGF-1 by dual-luciferase activity analysis. MTT assay revealed that miR-1 significantly inhibited the chondrocyte proliferation of deer antler tip. Furthermore, IGF-1 expression was decreased after transfection with miR-1 mimics by Western blotting. Therefore, miR-1 may be as a novel regulator of insulin-like growth factor-1 in deer antler proliferation and regeneration. Our study is the first investigation of the regulating effect of miR-1 on the expression of the IGF-1 gene in deer antler.
引用
收藏
页码：53 / 56
页数：4
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