Extractive Spectrophotometric for Determination of Dihydroergocryptine Mesylate in Pharmaceutical Preparations

Simple, accurate and highly sensitive spectrophotometric method has been developed for the rapid determination of dihydroergocryptine mesylate in pure form and pharmaceutical formulations. The spectrophotometric method was based on the formation of binary complex (ion-pair complex) between the dihydroergocryptine mesylate and chromotrope 2R in acidic buffer, giving purple color. The absorbance of dichloromethane extracted complex was measured at 531 nm. The effects of analytical parameters on the reported systems were investigated. The complexation reaction was extremely rapid at room temperature and the absorption value remains unchanged up to 24 h. Beer's law was obeyed in the concentration ranges of 3-180 mu g/mL, detection limit was 0.08 mu g/mL and the molar absorptivity coefficient were 6.8 x 103 L mol(-1) cm(-1) for chromotrope 2R. Recoveries were between 97.20-101.11%. Interferences of the other ingredients and excipients were not observed.