Comparison of biochemical and molecular genetic approaches for identification of environmental strains

被引：4

作者：

Korshunov, S. O. ^{[1
]}

Gorovtsov, A. V. ^{[1
]}

Faleeva, T. G. ^{[2
,3
]}

Gorbov, S. N. ^{[1
]}

Kornienko, I. V. ^{[1
,3
]}

机构：

[1] Southern Fed Univ, Rostov Na Donu 344006, Russia

[2] Rostov State Med Univ, Rostov Na Donu 344022, Russia

[3] Minist Def Russian Federat, Southern Mil Dist, State Ctr Forens Med & Criminal Expertise 16, Rostov Na Donu 344010, Russia

来源：

MICROBIOLOGY | 2014年 / 83卷 / 04期

关键词：

microorganisms; identification; 16S rRNA; biochemical characteristics; comprehensive approach; RIBOSOMAL-RNA GENE; 16S; BACTERIA; SYSTEM;

D O I：

10.1134/S0026261714040109

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, and morphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach-combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters-for identification of microorganisms isolated from environmental objects and clinical samples are discussed.

引用

页码：376 / 380

页数：5

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