The role of Beta-Proteobacteria in aromatic hydrocarbon degradation: fingerprinting of 16S rRNA gene and catechol 2,3-dioxygenase gene by T-RFLP in BTEX degradative bacterial communities

被引：4

作者：

Tancsics, Andras ^{[1
]}

Szabo, Istvan ^{[2
]}

Szoboszlay, Sandor ^{[2
]}

Baka, Erzsebet ^{[1
]}

Marialigeti, Karoly ^{[1
]}

Revesz, Sara ^{[1
]}

机构：

[1] Eotvos Lorand Univ, Dept Microbiol, Pazmany P Stny 1-C, H-1117 Budapest, Hungary

[2] Szent Istvan Univ, Dept Environm Protect & Environm Safety, H-2100 Godollo, Hungary

来源：

CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY | 2009年

关键词：

catechol 2,3-dioxygenase; BTEX degradation; T-RFLP; beta-Proteobacteria; SITE; PCR;

D O I：

10.1142/9789812837554_0138

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Detection of catechol 2,3-dioxygenase genes in aromatic hydrocarbon contaminated environments gives the opportunity to measure the diversity of bacteria involved in the degradation of these contaminants. In this study a primer set was designed to detect Comamonadaceae family (beta-Proteobacteria) related catechol 2,3-dioxygenase genes in BTEX contaminated groundwater and diversity of these genes was investigated by terminal restriction fragment length polymorphism. Major differencies were observed in the microbial structure between the contaminated and the non-contaminated groundwater samples.

引用

页码：664 / +

页数：2

共 4 条

[1] Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater
Tancsics, Andras
Szabo, Istvan
Baka, Erzsebet
Szoboszlay, Sandor
Kukolya, Jozsef
Kriszt, Balazs
Marialigeti, Karoly
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (07) : 398 - 406
[2] Vegetation cover of forest, shrub and pasture strongly influences soil bacterial community structure as revealed by 16S rRNA gene T-RFLP analysis
Chan, On Chim
Casper, Peter
Sha, Li Qing
Feng, Zhi Li
Fu, Yun
Yang, Xiao Dong
Ulrich, Andreas
Zou, Xiao Ming
FEMS MICROBIOLOGY ECOLOGY, 2008, 64 (03) : 449 - 458
[3] Bacterial diversity in livestock manure composts as characterized by terminal restriction fragment length polymorphisms (T-RFLP) of PCR-amplifled 16s rRNA gene sequences
Tiquia, SM
Michel, FC
MICROBIOLOGY OF COMPOSTING, 2002, : 65 - 82
[4] Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: Do the different methods provide similar results?
Smalla, Kornelia
Oros-Sichler, Miruna
Milling, Annett
Heuer, Holger
Baumgarte, Susanne
Becker, Regina
Neuber, Gabriele
Kropf, Siegfried
Ulrich, Andreas
Tebbe, Christoph C.
JOURNAL OF MICROBIOLOGICAL METHODS, 2007, 69 (03) : 470 - 479

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