Immobilization of His-tagged proteins through interaction with L-cysteine electrodeposited on modified gold surfaces

被引：2

作者：

Miyata, Hidefumi ^{[1
]}

Yumoto, Kazuhiro ^{[1
]}

Itoh, Kanako ^{[1
]}

Sasahara, Miki ^{[1
]}

Kawaura, Hiroki ^{[1
]}

Oshima, Nobuyuki ^{[1
]}

Shuzuki, Taiho ^{[1
]}

Takahashi, Shunsuke ^{[1
]}

Oshige, Masahiko ^{[1
]}

Katsura, Shinji ^{[1
]}

机构：

[1] Gunma Univ, Grad Sch Engn, Dept Chem & Environm Engn, Kiryu, Gunma 3768515, Japan

来源：

ADVANCED MICRO-DEVICE ENGINEERING IV | 2014年 / 596卷

关键词：

Immobilization; Green fluorescent protein; Electrodeposition; L-cysteine; SELF-ASSEMBLED MONOLAYERS; BIOSENSOR; BINDING;

D O I：

10.4028/www.scientific.net/KEM.596.219

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A method for preparing a high-density His-tagged protein array was developed. The method is based on specific binding between His-tags and Ni ions chelated with the carboxyl groups of L-cysteine applied to the substrate surface by electrodeposition. About 13 ng/mm(2) of His-tagged green fluorescent protein (His-GFP) could be immobilized on the substrate. The immobilized His-GFP could be subsequently released by washing with imidazole, suggesting that immobilization involves specific binding between the His-tag and the Ni ion complex.

引用

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页码：219 / 223

页数：5

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