Protective effect of liver ischemic preconditioning on rat hepatocytes

被引：5
|
作者：
Fu JianZhu [1 ,2 ]
Wang Yu [1 ]
Zhang LiJun [2 ]
Yu ZeLi [2 ]
机构：
[1] Capital Med Univ, Beijing Friendship Hosp, Dept Gen Surg, Beijing 100050, Peoples R China
[2] Capital Med Univ, Beijing Tongren Hosp, Dept Gen Surg, Beijing 100730, Peoples R China
来源：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 2009年 / 52卷 / 09期
关键词：
liver; ischemic preconditioning; hepatocytes; cryopreservation; REPERFUSION INJURY; MOUSE-LIVER; TRANSPLANTATION; RESECTION; MICROCIRCULATION; APOPTOSIS; MODEL; TRIAL;
D O I：
10.1007/s11427-009-0113-5
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Ischemic preconditioning (IPC) protects liver graft function following ischemia in liver transplantation and liver resection. The aim of this study was to assess the advantages and any potential disadvantages of liver IPC prior to isolation for rat hepatocytes during isolation and cryopreservation. After isolating and thawing the cryopreserved hepatocytes after 14 and 28 d, cell viability, efficiency, and lactate dehydrogenase (LDH) levels in preserve solution were examined for every group. Groups treated with IPC had better cell viability determination, assessment of plating efficiency and lactate dehydrogenase (LDH) assay than Group without IPC, suggesting that IPC prior to isolation may have a significant protective effect on hepatocytes subjected to isolation and short period cryopreservation.
引用
收藏
页码：836 / 840
页数：5
相关论文
共 50 条
  • [1] Protective effect of liver ischemic preconditioning on rat hepatocytes
    JianZhu Fu
    Yu Wang
    LiJun Zhang
    ZeLi Yu
    Science in China Series C: Life Sciences, 2009, 52 : 836 - 840
  • [2] Protective effect of liver ischemic preconditioning on rat hepatocytes
    FU JianZhu1
    2 Department of General Surgery
    Science China Life Sciences, 2009, (09) : 836 - 840
  • [3] Protective effect of ischemic preconditioning on liver
    张燕忠
    张柏根
    潘仁龙
    中华创伤杂志(英文版), 2001, (02) : 59 - 61
  • [4] Protective effect of ischemic preconditioning on liver
    张燕忠
    张柏根
    潘仁龙
    ChineseJournalofTraumatology, 2001, (02)
  • [5] The protective effect of ischemic preconditioning on rat testis
    Sahinkanat, Tayfun
    Ozkan, K. Ugur
    Tolun, Fatma I.
    Ciralik, Harun
    Imrek, Secil S.
    REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2007, 5 (1)
  • [6] The protective effect of ischemic preconditioning on rat testis
    Tayfun Sahinkanat
    K Ugur Ozkan
    Fatma I Tolun
    Harun Ciralik
    Secil S Imrek
    Reproductive Biology and Endocrinology, 5
  • [7] Reperfusion injury of the liver:: Protective effect of ischemic preconditioning
    Cutrìn, JC
    Poli, G
    FREE RADICAL RESEARCH, 2003, 37 : 24 - 24
  • [8] Reperfusion injury of the liver:: Protective effect of ischemic preconditioning
    Cutrìn, JC
    Cavalieri, B
    Perreli, MG
    Ramadori, P
    Durazzo, M
    Poli, G
    PROCEEDINGS OF THE MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH, EUROPEAN SECTION: FREE RADICALS AND OXIDATIVE STRESS: CHEMISTRY, BIOCHEMISTRY AND PATHOPHYSIOLOGICAL IMPLICATIONS, 2003, : 225 - 232
  • [9] Synergistic protective effect of ischemic preconditioning and allopurinol on ischemia/reperfusion injury in rat liver
    Lee, Woo-Yong
    Lee, Sun-Mee
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 349 (03) : 1087 - 1093
  • [10] Protective effect of ischemic preconditioning against hepatic ischemic injury during the course of liver resection in a rat model
    Abdeldayem, Hesham
    Ahmad-El-Refaie, Ahmad
    Bassiouny, Ashraf
    VIRCHOWS ARCHIV, 2007, 451 (02) : 212 - 212
12345