Identification of non-tuberculous mycobacteria: 16S rRNA gene sequence analysis vs. conventional methods

被引：0
|
作者：
El Amin, NM
Hanson, HS
Pettersson, B
Petrini, B [1 ]
Von Stedingk, LV
机构：
[1] Karolinska Hosp & Inst, Dept Clin Microbiol, SE-17176 Stockholm, Sweden
[2] Natl TB Programme, Khartoum, Sudan
[3] Huddinge Hosp, IMPI, Div Clin Bacteriol, Huddinge, Sweden
[4] Sahlgrens Univ Hosp, Dept Clin Bacteriol, S-41345 Gothenburg, Sweden
来源：
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES | 2000年 / 32卷 / 01期
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D O I：
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中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In a retrospective study 45 clinical isolates of non-tuberculous mycobacteria were identified to the species level by biochemical profile, gas liquid chromatography and partial sequence analysis of 16S rRNA, and were found to represent 13 different species. The results of sequence analysis showed 100% identity with conventional tests for 34 isolates (76%) and could identify species such as M. bohemieum which are difficult to characterise with conventional methods. Most of the discrepant results for the remaining 11 isolates resulted in species of the same group of mycobacteria. Based on these findings, we concluded that direct sequence analysis of amplified 16S rRNA gene is a promising rapid and accurate method for species determination of non-tuberculous mycobacteria.
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页码：47 / 50
页数：4
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