Human Plasma Protein Adsorption on Carbon-Based Materials

被引:8
|
作者
Vinante, Michele [1 ]
Digregorio, Gabriella [2 ]
Lunelli, Lorenzo [1 ]
Forti, Stefania [1 ]
Musso, Simone [2 ]
Vanzetti, Lia [1 ]
Lui, Alberto [1 ]
Pasquardini, Laura [1 ]
Giorcelli, Mauro [2 ]
Tagliaferro, Alberto [2 ]
Anderle, Mariano [1 ]
Pederzolli, Cecilia [1 ]
机构
[1] FBK Irst, I-38050 Povo, TN, Italy
[2] Politecn Torino, Dept Phys, I-10129 Turin, Italy
关键词
Nanostructured Carbon; Hemocompatibility; Fibrinogen; Hageman Factor; Surface Analysis; SELF-ASSEMBLED MONOLAYERS; FIBRINOGEN ADSORPTION; SURFACES; NANOTUBES; CVD; GROWTH; VALVE;
D O I
10.1166/jnn.2009.NS68
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this study the initial reactions of different carbon-based materials with human blood were investigated by short-time exposure to platelet poor plasma (PPP). Extent of protein adsorption and conformational changes of proteins adsorbed on material surfaces are known to be keys factors affecting further biological reactions. Plasma protein adsorption on multi-walled carbon nanotubes (MWCNTs), highly oriented pyrolytic graphite (HOPG) and nanocrystalline graphite (NG) were investigated and the results obtained on these materials were compared with those obtained studying pyrolytic carbon (PyC), a material showing good anti-trombogenic properties. The quantification of adsorbed plasma proteins on sample surfaces was obtained by Micro BCA Protein Assay, while immunofluorescence analysis was employed to monitor the surface density and distribution of two selected proteins, namely fibrinogen (Fg) and Hageman factor (FXII), proteins playing a leading role in mediating platelet adhesion. The dependence of the biological response on the surface chemical and morphological properties were also investigated and data obtained using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) are presented. After PPP incubation PyC is characterized by the presence of low level of whole proteins and FXII adsorption, in contrast to a high adhesion of Fg. Compared to PyC the analysis of the other carbon-based materials result in a higher whole protein adsorption with an increasing trend moving from MWCNTs, NG and HOPG respectively. The Fg surface density on PyC, NG and MWCNTs is about four times higher than on HOPG while only HOPG show a detectable fluorescent signal of FXII. If AFM data indicate that surface morphology does not play a crucial role in protein adhesion, XPS analysis show chemical differences that can be correlated with this biological response.
引用
收藏
页码:3785 / 3791
页数:7
相关论文
共 50 条
  • [1] Plasma production in carbon-based materials
    Giuffreda, E.
    Delle Side, A.
    Nassisi, V.
    Krasa, J.
    [J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2017, 406 : 225 - 228
  • [2] Adsorption of Bovine Serum Albumin on Carbon-Based Materials
    Seredych, Mykola
    Mikhalovska, Lyuba
    Mikhalovsky, Sergey
    Gogotsi, Yury
    [J]. C-JOURNAL OF CARBON RESEARCH, 2018, 4 (01):
  • [3] Adsorption characteristics of triclosan and naproxen in water by carbon-based materials
    Deng, Jing
    Lu, Yuan
    Xiao, Zijie
    Zhang, Tuqiao
    Li, Jun
    Ni, Yongjiong
    Ma, Xiaoyan
    [J]. Zhongnan Daxue Xuebao (Ziran Kexue Ban)/Journal of Central South University (Science and Technology), 2016, 47 (04): : 1427 - 1435
  • [4] Nanoscale carbon-based materials in protein isolation and preconcentration
    Chen, Xuwei
    Hu, Linlin
    Liu, Jiawei
    Chen, Shuai
    Wang, Jianhua
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2013, 48 : 30 - 39
  • [5] Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials
    Zonglin Gu
    Zaixing Yang
    Yu Chong
    Cuicui Ge
    Jeffrey K. Weber
    David R. Bell
    Ruhong Zhou
    [J]. Scientific Reports, 5
  • [6] Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials
    Gu, Zonglin
    Yang, Zaixing
    Chong, Yu
    Ge, Cuicui
    Weber, Jeffrey K.
    Bell, David R.
    Zhou, Ruhong
    [J]. SCIENTIFIC REPORTS, 2015, 5
  • [7] Carbon-based materials in steady state flow of deuterium plasma
    Antonov, NV
    Kornienko, SN
    Litnovsky, AM
    Petrov, VB
    Pleshakov, AS
    Khripunov, BI
    Shapkin, VV
    [J]. IZVESTIYA AKADEMII NAUK SERIYA FIZICHESKAYA, 2000, 64 (04): : 796 - 800
  • [8] Machine learning for the selection of carbon-based materials for tetracycline and sulfamethoxazole adsorption
    Zhu, Xinzhe
    Wan, Zhonghao
    Tsang, Daniel C. W.
    He, Mingjing
    Hou, Deyi
    Su, Zhishan
    Shang, Jin
    [J]. CHEMICAL ENGINEERING JOURNAL, 2021, 406 (406)
  • [9] Recent advances on activated carbon-based materials for nitrate adsorption: A review
    Ahmed, M. J.
    Hameed, B. H.
    Khan, M. A.
    [J]. JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS, 2023, 169
  • [10] ADSORPTION CHARACTERISTICS OF NITRIC OXIDE ONTO POROUS CARBON-BASED MATERIALS
    Kang, Dongjuan
    Tang, Xiaolong
    Peng, Jinhui
    Yi, Honghong
    Ning, Ping
    Ye, Zhiqing
    Li, Kai
    [J]. FRESENIUS ENVIRONMENTAL BULLETIN, 2011, 20 (10): : 2562 - 2568