Semi-automated methodology for detection of IgM oligoclonal bands in cerebrospinal fluid and serum samples

被引：3
|
作者：
Cabrera, Carmen M. [1 ]
Gosis, Andrea [2 ]
机构：
[1] Hosp Gen Univ Ciudad Real, Immunol Sect, C Obispo Rafael Torija S-N, Ciudad Real 13005, Spain
[2] Carlos Haya Reg Hosp, Neurol Serv, Malaga, Spain
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2020年 / 487卷
关键词：
Cerebrospinal fluid; Hydrasys platform; IgM oligoclonal bands; Isoelectrofocusing; Multiple sclerosis; Semi-automated methodology; MULTIPLE-SCLEROSIS; IMMUNOGLOBULIN-M; PREDICTS;
D O I：
10.1016/j.jim.2020.112898
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Among the new biomarkers to propose therapeutic decisions in patients suffering from multiple sclerosis (MS) are the IgM oligoclonal bands (OCBs) in cerebrospinal fluid (CSF). At the current time, however, IgM OCBs are detected in laboratories at investigation level and not in the routine practice due to their complexity. For this, we have applied a semi-automated method based on an isoelectrofocusing platform from Sebia of wide availability in clinical laboratories. The IgM OCBs results were validated in paired samples of CSF and serum from patients with MS previously carried out in a reference laboratory. We found a sensitivity of 91.67%, in agreement with previous data obtained with the reference method for IgM OCBs.
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页数：3
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