Reversed-Phase Liquid Chromatographic Profile of Free Amino Acids in Strawberry-tree (Arbutus unedo L.) Honey

被引：0

作者：

Spano, Nadia ^{[1
]}

Piras, Irene ^{[1
]}

Ciulu, Marco ^{[1
]}

Floris, Ignazio ^{[2
]}

Panzanelli, Angelo ^{[1
]}

Pilo, Maria I. ^{[1
]}

Piu, Paola C. ^{[1
]}

Sanna, Gavino ^{[1
]}

机构：

[1] Univ Sassari, Dipartimento Chim, I-07100 Sassari, Italy

[2] Univ Sassari, Dipartimento Protez Piante, I-07100 Sassari, Italy

来源：

JOURNAL OF AOAC INTERNATIONAL | 2009年 / 92卷 / 04期

关键词：

RAPID-DETERMINATION; HOMOGENTISIC ACID; BOTANICAL ORIGIN; DERIVATIZATION; PHENYLISOTHIOCYANATE; SAMPLES;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The typical profile of the free amino acids contained in strawberry-tree (Arbutus unedo L.) honey is reported for the first time. An optimized reversed-phase liquid chromatographic (RP-LC) method with phenyl isothiocyanate precolumn derivatization was used. Fourteen free amino acids were identified and quantified in 16 analytical samples. Proline (65.63%) was found to be the most abundant free amino acid, followed by glutamic acid (6.49%), arginine (5.21%), alanine (5.17%), and phenylalanine (4.97%). The total free amino acid content of strawberry-tree honey (average value, 436 mg/kg) was found to be low in comparison to amounts cited in the literature concerning unifloral honeys. The analytical method was optimized and fully validated in terms of detection and quantitation limits, precision (by testing repeatability and reproducibility), linearity, and bias (by means of recovery tests). The acceptability of the validation protocol results was verified using Horwitz's mathematical model and AOAC guidelines.

引用

页码：1145 / 1152

页数：8

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