The study of benthic macrofaunal communities along the NW coasts of Rodos (Rhodes) Island indicates that the area can be characterized as subtropical, with a high-energy hydrological regime. Of five stations sampled, 382 taxa were identified, some of special zoogeographic interest. The high diversity values observed are probably related to the rather coarse sediment (muddy sand) even in the deeper parts. No clear zonation of species composition was observed, while a very large number of rare species were found. Contribution of Lessepsian migrants to the benthic community composition is very low. Sorting coefficients of the sediment, depth, and hydrological conditions seem to play a synergistic role in the discrimination of benthic assemblages.