In situ hybridization analysis in IgA nephropathy

In situ hybridization provides much information on gene expression in renal biopsy specimens and is useful for detecting intrarenal cells producing molecules that are also present in the circulation. These molecules may deposit in the glomeruli. In situ hybridization is also useful for detecting cells producing cytokine that may be secreted immediately from such cells. Finally, in situ hybridization is useful for detecting extracellular matrix producing cells. Semiquantitative in situ hybridization is useful for assessing the retained RNA in the tissue and comparing the expression of specific mRNA in different tissues. ?his method may be useful in predicting the clinical outcome at the time of renal biopsy. In conclusion, in situ hybridization allows for the evaluation of cell function in human renal biopsy specimens. It not only provides information related to cell morphology, but also provides an estimation of functionality of human renal biopsy specimens.