Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal alkaline phosphatase

被引：2
|
作者：
Craig, Douglas B. [1 ]
Hanlon-Dearman, Fiona [1 ]
Beaudry, Shailah [1 ]
Shek, Kevin [1 ]
King, Steffany D. [1 ]
机构：
[1] Univ Winnipeg, Dept Chem, Winnipeg, MB R3B 2E9, Canada
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2015年 / 114卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
Alkaline phosphatase; Bovine; Pichia pastoris; Recombinant; Single molecule; COLI BETA-GALACTOSIDASE; INDIVIDUAL MOLECULES; ENZYME MOLECULES;
D O I：
10.1016/j.pep.2015.06.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Single molecule assays were performed on bovine intestinal alkaline phosphatase and the recombinant enzyme expressed in Pichia pastoris using a capillary electrophoresis-based method. The catalytic rates for the bovine and recombinant enzymes were found to be 11,000 +/- 7000 min(-1) (N = 161) and 12,000 +/- 7000 min(-1) (N = 173), respectively. Mean catalytic rates and variances did not differ significantly between the enzyme from both sources. Furthermore, the distribution of catalytic rates were indistinguishable. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：95 / 98
页数：4
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