β- Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2

被引：38

作者：

Zhao, Yuan ^{[1
,2
]}

Qin, Guixin ^{[1
,2
]}

Han, Rui ^{[1
]}

Wang, Jun ^{[1
]}

Zhang, Xiaodong ^{[3
]}

Liu, Dandan ^{[1
]}

机构：

[1] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China

[2] Jilin Agr Univ, Minist Educ, Key Lab Anim Prod Prod Qual & Secur, Changchun 130118, Peoples R China

[3] Jilin Univ, Coll Vet Med, Key Lab Zoonosis Res, Inst Zoonosis,Minist Educ, Changchun 130062, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2014年 / 15卷 / 02期

基金：

中国国家自然科学基金;

关键词：

-conglycinin; intestinal barrier; tight junction; food allergen; IPEC-J2; INTESTINAL ALKALINE-PHOSPHATASE; TRANSEPITHELIAL ANTIGEN TRANSPORT; EARLY-WEANED PIG; FOOD ALLERGY; IN-VITRO; SOYBEAN-MEAL; PERMEABILITY; MODEL; HYPERSENSITIVITY; CELLS;

D O I：

10.3390/ijms15021915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen -conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by -conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing -conglycinin (p < 0.001).

引用

页码：1915 / 1926

页数：12

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