Studying the impact of S9 on cyto-genotoxicity of cigarette smoke in human peripheral blood lymphocytes in vitro

被引：6

作者：

Lou Jianlin ^{[1
,2
,3
]}

Chu Guohai ^{[3
]}

Zhou Guojun ^{[3
]}

Jiang Jian ^{[3
]}

Huang Fangfang ^{[3
]}

Xu Juanjuan ^{[1
]}

Zheng Shu ^{[2
]}

Chen Zhijian ^{[1
]}

Jiang Wei ^{[1
]}

Lu Yezhen ^{[1
]}

Li Xiaoxue ^{[1
]}

He Jiliang ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Med, Inst Environm Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Coll Med, Affiliated Hosp 2, Inst Oncol, Hangzhou 310002, Zhejiang, Peoples R China

[3] China Tobacco Zhejiang Ind Co Ltd, Ctr Technol, Hangzhou 310008, Zhejiang, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2009年 / 28卷 / 02期

关键词：

Apoptosis; CCK-8; assay; Micronucleus assay; DNA repair; Cigarette smoke condensates; TOBACCO-SMOKE; COMET ASSAY; CELLS; CONDENSATE; INDUCTION; APOPTOSIS; CHEMICALS; STRESS;

D O I：

10.1016/j.etap.2009.05.001

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In present study, human lymphocytes were exposed to cigarette smoke condensates (CSCs) at the doses of 25, 50, 75, 100 and 125 mu g ml(-1) with and without S9, and the cyto-genotoxic effects were detected with CCK-8, cell apoptosis and micronucleus assays. DNA repair kinetics was observed with comet assay. Our results indicated that the cell viability decreased with CSCs doses, the percentages of apoptosis cell and the frequencies of micronuclei increased with CSCs doses, and DNA damage of human lymphocytes induced by CSCs could be basically repaired within 240 min. However, the cytotoxicity induced by CSCs +S9 was significantly lower than that induced by CSCs -S9 in CCK-8 and cell apoptosis assays, and the DNA repair speed in +S9 group was quicker than that in -S9 group. In conclusion, S9 may affect not only the cyto-genotoxicity of CSCs but also the repair process of DNA damage induced by CSCs in lymphocytes. (C) 2009 Published by Elsevier B.V.

引用

页码：275 / 279

页数：5

共 25 条

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