A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

被引：55
|
作者：
Moury, B [1 ]
Pflieger, S [1 ]
Blattes, A [1 ]
Lefebvre, V [1 ]
Palloix, A [1 ]
机构：
[1] INRA, Unite Genet & Ameliorat Fruits & Legumes, F-84143 Montfavet, France
来源：
GENOME | 2000年 / 43卷 / 01期
关键词：
Capsicum sp; PCR marker; bulked segregant analysis; Tospovirus; resistance gene;
D O I：
10.1139/gen-43-1-137
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F-2 individuals. A close RAPD marker was converted into a codominant cleaved amplified polymorphic sequence (CAPS) using specific PCR primers and restriction enzymes. This CAPS marker is tightly linked to Tsw (0.9 +/- 0.6 cM) and is helpful for marker-assisted selection in a wide range of genetic intercrosses.
引用
收藏
页码：137 / 142
页数：6
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