COMPARISON OF ANTIMICROBIAL, ANTIOXIDANT CAPACITIES AND HPLC ANALYSIS OF THREE Stachys SPECIES IN TURKEY

Antimicrobial and antioxidant activities of three Stachys species, S. balansae subsp. balansae, S. mardinensis and S. megalodonta subsp. mardinensis, were evaluated. The antimicrobial activity of methanol extracts were determined by minimum inhibitory concentrations (MIC) method, the best activity (MIC value of 1000 mu g/ml) was observed with S. megalodonta subsp. mardinensis against E. coli and S. aureus. Antioxidant activities of the methanol extract from Stachys species were tested by the DPPH radical scavenging assay, the metal chelating activity assay and beta-carotene-lycopene, total phenol, total flavonoid contents were determined. In addition, flavonoids content was analyaed by HPLC. The highest phenolic content was found in S. mardinensis (225.10 mg GAE/g), however, the highest flavonoid content was found in S. megalodonta subsp. mardinensis (79.09 mg QE/g). According to the HPLC flavonoid analysis results, S. mardinensis had higher quercetin (26.5 mg/g) and catechine (11.00 mg/g) contents than the other Stachys species.