Comparison of analytical methods and the influence of milk components on milk urea nitrogen recovery

The objectives of this study were to compare analytical instruments used in independent laboratories to measure milk urea nitrogen (MUN) and determine whether any components in milk affect the recovery of MUN. Milk samples were collected from 100 Holstein cows fed one ration in a commercial dairy herd with a rolling herd average of 9500 kg. Half of each sample was spiked with 4 mg/dL of urea N, while the other half was not, to determine recovery. Both milk samples ( spiked and not spiked) were sent to 14 independent laboratories involved in the MUN Quality Control Program through National Dairy Herd Improvement Association and analyzed for MUN, fat, protein, lactose, somatic cell count (SCC), and total solids. The laboratories analyzed MUN using CL-10 (n = 3), Skalar ( n = 2), Bentley ( n = 3), Foss 4000 ( n = 3) or Foss 6000 ( n = 3) systems. When recovery of MUN was evaluated among the 5 analytical methods, the mean recoveries for the Bentley, Foss 6000, and Skalar systems were 92.1 (SE = 2.76%), 95.4 (SE = 10.1%), and 95.1% (SE = 7.61%), respectively, and did not differ from each other. However, MUN recovery was 85.0% (SE = 2.8%) for the CL-10 system and 47.1% (SE = 9.9%) for the Foss 4000 system, both of which differed from the other 3 systems. Recoveries from Foss 4000, Foss 6000, and Skalar varied among laboratories using the same instrument. As initial MUN concentration increased, recovery decreased using the Bentley and CL-10 systems. Increasing milk fat resulted in a decrease in recovery using the Foss 6000 system. For 4 of the 5 methods, recovery of MUN was not associated with specific milk components. Recovery of MUN was inconsistent for laboratories using the Foss 4000 and the Foss 6000 method and using these systems may result in an overestimation or underestimation of MUN.