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DNA Barcoding of Simulium asakoae (Diptera: Simuliidae) From Northern Thailand
被引:10
|作者:
Low, Van Lun
[1
]
Srisuka, Wichai
[2
]
Saeung, Atiporn
[3
]
Tan, Tiong Kai
[4
]
Ya'cob, Zubaidah
[1
]
Yeong, Yze Shiuan
[1
]
Takaoka, Hiroyuki
[1
]
机构:
[1] Univ Malaya, Trop Infect Dis Res & Educ Ctr TIDREC, Kuala Lumpur, Malaysia
[2] Queen Sirikit Bot Garden, Entomol Sect, POB 7, Chiang Mai, Thailand
[3] Chiang Mai Univ, Fac Med, Dept Parasitol, Chiang Mai, Thailand
[4] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur, Malaysia
关键词:
DNA barcode;
COI gene;
black fly;
vector;
Thailand;
MALAYSIA;
D O I:
10.1093/jme/tjaa081
中图分类号:
Q96 [昆虫学];
学科分类号:
摘要:
Previous studies suggested the presence of species complex in the so-called Simulium asakoaeTakaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present inThailand, we performed a detailed morphological identification of S. asakoae and compared its DNA barcodes with the morphospecies S. asakoae from Myanmar and the typical S. asakoae from Malaysia. Phylogenetic analysis revealed theThai materials analyzed in this study were indeed genetically similar with those from Myanmar and Malaysia, though genetic distances 0-2.27% were observed. We tentatively regard this divergence as intraspecific variation, and the automatic barcode gap discovery analysis further supports them as a single species.
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页码:1675 / 1678
页数:4
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