Expression systems for production of recombinant allergens

被引：41
|
作者：
Schmidt, M [1 ]
Hoffman, DR [1 ]
机构：
[1] E Carolina Univ, Dept Biol, Greenville, NC 27858 USA
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 2002年 / 128卷 / 04期
关键词：
allergen; prokaryotic systems; eukaryotic systems; baculovirus; yeast; plant; recombinant allergens;
D O I：
10.1159/000063865
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Recombinant allergenic proteins have been produced in a variety of different expression systems. This review gives examples of and compares prokaryotic expression systems, such as Escherichia coli, and eukaryotic systems including the yeasts, Saccharomyces cerevisiae and Pichia pastoris, baculovirus-insect cell systems, mammalian cell systems and plant systems. Important factors to consider in choosing an expression system are discussed and examples of applications are given. Copyright (C) 2002 S. Karger AG, Basel.
引用
收藏
页码：264 / 270
页数：7
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