Enzymatic activity of lipase entrapped in CTAB/water/pentanol/hexane reverse micelles: a functional and microstructural investigation

被引：6
|
作者：
Lopez, F [1 ]
Palazzo, G [1 ]
Colafemmina, G [1 ]
Cinelli, G [1 ]
Ambrosone, L [1 ]
Ceglie, A [1 ]
机构：
[1] Univ Molise, Dept Food Technol, Consorzio Interuniv Sviluppo Sistemi Grande Inter, CSGI, I-86100 Campobasso, Italy
来源：
TRENDS IN COLLOID AND INTERFACE SCIENCE XVI | 2004年 / 123卷
关键词：
reverse micelles; CTAB; pentanol; lipase;
D O I：
10.1007/b11756
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The lipase VII (from Candida rugosa) activity was studied as a function of the content of pentanol and water in cethyltrimethylammonium. bromide (CTAB)/ water/pentanol/hexane reverse micelles. The turn-over numbers for the hydrolysis of p-nitrophenyl butyrate (p-NPB) were determined spectrophotometrically. Reverse micelles size and the partition of p-NPB between the aggregates and the oil continuous phase were determined by means of pulsed gradient spin-echo NMR experiments. A strong correlation between enzyme activity and micellar size was found.
引用
收藏
页码：174 / 177
页数：4
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