Interspecies discrimination of A-fumigatus and siblings A-lentulus and A-felis of the Aspergillus section Fumigati using the AsperGenius® assay

被引：16

作者：

Chong, G. M. ^{[1
]}

Vonk, A. G. ^{[2
]}

Meis, J. F. ^{[3
,4
]}

Dingemans, G. J. H. ^{[5
]}

Houbraken, J. ^{[6
]}

Hagen, F. ^{[3
]}

Gaajetaan, G. R. ^{[5
]}

van Tegelen, D. W. E. ^{[5
]}

Simons, G. F. M. ^{[5
]}

Rijnders, B. J. A. ^{[1
]}

机构：

[1] Erasmus Univ, Med Ctr, Infect Dis Sect, Dept Internal Med, Rotterdam, Netherlands

[2] Erasmus Univ, Med Ctr, Dept Med Microbiol, Rotterdam, Netherlands

[3] Canisius Wilhelmina Hosp CWZ, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands

[4] Radboudumc, Dept Med Microbiol, Nijmegen, Netherlands

[5] PathoNostics BV, Maastricht, Netherlands

[6] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 87卷 / 03期

关键词：

Aspergillus fumigatus; Aspergillus lentulus; Aspergillus felis; Cryptic species; Sibling species; Polymerase chain reaction; INVASIVE ASPERGILLOSIS; AZOLE RESISTANCE; BRONCHOALVEOLAR LAVAGE; DEMETHYLASE CYP51A; ANTIFUNGAL THERAPY; DISEASE; PATIENT; VIRIDINUTANS; VALIDATION; LEUKEMIA;

D O I：

10.1016/j.diagmicrobio.2016.11.020

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The AsperGenius (R) assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/ T289A/Y121F that are associated with azole resistance. We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling species within the Aspergillus section Fumigati. Identification of these species with conventional culture techniques is difficult and time-consuming. The assay was tested on (i) 2 A. lentulus and A. felis strains obtained from biopsy proven invasive aspergillosis and (ii) control A. fumigatus (n = 3), A. lentulus (n = 6) and A. felis species complex (n = 12) strains. The AsperGenius (R) resistance PCR did not detect the TR34 target in A. lentulus and A. fells in contrast to A. fumigatus. Melting peaks for L98H and Y121F markers differed and those of the Y121F marker were particularly suitable to discriminate the 3 species. In conclusion, the assay can be used to rapidly discriminate A. fumigatus, A. lentulus and A. fells. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license.

引用

页码：247 / 252

页数：6