Biodegradation of triazine herbicides on polyvinylalcohol gel plates by the soil yeast Lipomyces starkeyi

被引：26

作者：

Nishimura, K ^{[1
]}

Yamamoto, M ^{[1
]}

Nakagomi, T ^{[1
]}

Takiguchi, Y ^{[1
]}

Naganuma, T ^{[1
]}

Uzuka, Y ^{[1
]}

机构：

[1] Yamanashi Univ, Fac Engn, Dept Appl Chem & Biotechnol, Kofu, Yamanashi 4008511, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 58卷 / 06期

关键词：

D O I：

10.1007/s00253-002-0950-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The soil yeast Lipomyces starkeyi was tested for its ability to degrade triazine herbicides. Polyvinyl-alcohol (PVA) was employed as a solid medium in culture plates instead of agar. The cell sizes of the control (without nitrogen source) on the PVA gel plate were much smaller than those on the agar gel plate. The difference between the diameters of the sample and control colonies on the PVA gel plate were almost twice those of the colonies on the agar gel plate (1.9 and 1.0 mm, respectively). Thus, the PVA gel plate is much better than the agar plate for evaluating the degree of utilization of a sole nitrogen source. The yeast grew well (more than 4 mm in diameter) with 1,3,5-triazine or cyanuric acid as nitrogen source. In addition, melamine and thiocyanuric acid inhibited growth of the yeast. and the sizes of colonies were smaller than those of the control. All triazine herbicides tested (simazine, atrazine, cyanazine, ametryn, and prometryn) could be degraded and assimilated by L. starkeyi.

引用

页码：848 / 852

页数：5