Condensation nucleation light scattering detection with microbore liquid chromatography for lipid analysis

被引：0
|
作者：
Yang, XH [1 ]
Koropchak, JA [1 ]
机构：
[1] So Illinois Univ, Dept Chem & Biochem, Carbondale, IL 62901 USA
来源：
JOURNAL OF MICROCOLUMN SEPARATIONS | 2000年 / 12卷 / 04期
关键词：
D O I：
10.1002/(SICI)1520-667X(2000)12:4<204::AID-MCS5>3.0.CO;2-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Methods of analysis for both neutral and phospholipids based on microbore high-pressure liquid chromatography HPLC coupled with detection by condensation nucleation light scattering detection are described. The limits of detection for phospholipids were below 100 ng/mL, corresponding to less than 0.5 ng or less than 1 pmole, but were even lower for neutral lipids, and down to 12 ng/mL (0.06 ng, 0.074 pmole). The method was applied to the determination of lipids in egg yolk samples. (C) 2000 John Wiley & Sons, Inc.
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页码：204 / 210
页数：7
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