Compact microfluidic device for rapid concentration of PET tracers

被引:14
|
作者
Tseng, Wei-Yu
van Dam, R. Michael [1 ]
机构
[1] Univ Calif Los Angeles, Crump Inst Mol Imaging, Los Angeles, CA 90095 USA
关键词
POSITRON-EMISSION-TOMOGRAPHY; RADIOSYNTHESIS; PRESSURE; CHIP;
D O I
10.1039/c4lc00286e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
HPLC purification and reformulation of positron emission tomography (PET) tracers can lead to significant dilution of the final product, making it difficult to produce a sufficiently high radioactivity concentration for some applications (e.g. small animal imaging, in vitro assays, and labelling of proteins with prosthetic groups). This is especially true for molecules with lengthy or low-yield syntheses. Starting the synthesis with more radioactivity increases the final radioactivity concentration but increases hazards and complexity of handling. An alternative is to concentrate the final product by a process such as rotary evaporation prior to downstream use. Because a rotovap requires significant space within a hot cell that could be put to more productive use, we developed a compact microfluidic system for concentration of PET tracers. This system also provides advantages in terms of repeatability, interfacing and potential for automation. We present here the design and performance characterization of the system, and demonstrate the concentration of several tracers in aqueous-based HPLC mobile phases.
引用
收藏
页码:2293 / 2302
页数:10
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