A small, cysteine-rich protein secreted by Fusarium oxysporum during colonization of xylem vessels is required for I-3-mediated resistance in tomato

被引：295
|
作者：
Rep, M
van der Does, HC
Meijer, M
van Wijk, R
Houterman, PM
Dekker, HL
de Koster, CG
Cornelissen, BJC
机构：
[1] Univ Amsterdam, Swammerdam Inst Life Sci, NL-1090 GB Amsterdam, Netherlands
[2] Univ Amsterdam, Swammerdam Inst Life Sci, NL-1018 WV Amsterdam, Netherlands
来源：
MOLECULAR MICROBIOLOGY | 2004年 / 53卷 / 05期
关键词：
D O I：
10.1111/j.1365-2958.2004.04177.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 12 kDa cysteine-rich protein is secreted by Fusarium oxysporum f. sp. lycopersici during colonization of tomato xylem vessels. Peptide sequences obtained with mass spectrometry allowed identification of the coding sequence. The gene encodes a 32 kDa protein, designated Six1 for (s) under bar ecreted (i) under barn (x) under bar ylem 1. The central part of Six1 corresponds to the 12 kDa protein found in xylem sap of infected plants. A mutant that had gained virulence on a tomato line with the I-3 resistance gene was found to have lost the SIX1 gene along with neighbouring sequences. Transformation of this mutant with SIX1 restored avirulence on the I-3 line. Conversely, deletion of the SIX1 gene in a wild-type strain results in breaking of I-3-mediated resistance. These results suggest that I-3-mediated resistance is based on recognition of Six1 secreted in xylem vessels.
引用
收藏
页码：1373 / 1383
页数：11
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