THE INFLUENCE OF VESSEL POLYPEPTIDE COMPLEX ON THE VIABILITY OF RATS UNDER COLD STRESS

The study of the mechanisms of preserving human viability in deep hypothermia, as well as the resilience of elderly people with hypothermia, is an urgent problem of physiology and medicine. The aim of the work is to study the effectiveness of the use of the vessels polypeptide complex (VPC) to stimulate the functions of the respiratory and cardiovascular systems in violation of life stability in the model of experimental hypothermia. Wistar's line rats were divided into an experimental group (injection of VPC) and a control group (injection of saline solution). VPC was administered intraperitoneal to rats at a dosage of 15.6 mg/kg of animal body weight 1 hour before the animals were immersed in a bath with cold water. The breath rate, heart rate, rectal temperature, esophageal temperature, and the degree of saturation of arterial hemoglobin with oxygen were evaluated. The heart rate in the rectal temperature range of 30-16 degrees C was significantly higher after the use of VPC in comparison with the control. The breath rate began to decrease at a rectal temperature of 27.7 +/- 0.3 oC during the cooling process in rats, which obtained VPC. A decrease in breath rate in the control group was observed, starting with a higher rectal temperature - 30.4 +/- 0.4 oC. The threshold for stopping breathing after the use of VPS was 11.6 +/- 0.3 oC for rectal temperature and 14.1 +/- 0.3 oC for esophageal temperature. In the control, these indicators were equal, respectively, 16.0 +/- 0.3 oC and 19.1 +/- 0.3 oC, which is 4-5 oC higher. The cooling time of rats to respiratory arrest increased by 2 times after the use of VPC in comparison with the control. It can be assumed that VPC slows down the process of cold respiratory depression and cardiac activity. The obtained results indicate the ability of the VPC to maintain the functions of the respiratory and cardiovascular systems for a long time in case of a violation of life stability in the model of experimental hypothermia. This indicates the prospects for further investigation of the VPC geroprotective properties and its ability to increase the cold resistance of the body in hypothermia.