Development of a fast responsive and highly sensitive fluorescent probe for Cu2+ ion and imaging in living cells

被引：38
|
作者：
Huang, Jiaguo [1 ]
Tang, Min [2 ]
Liu, Min [1 ]
Zhou, Ming [1 ]
Liu, Zhiguo [1 ]
Cao, Ya [2 ]
Zhu, Meiyan [1 ]
Liu, Shaogang [3 ]
Zeng, Wenbin [1 ]
机构：
[1] Cent S Univ, Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China
[2] Cent S Univ, Canc Res Inst, Changsha 410013, Hunan, Peoples R China
[3] Cent S Univ, NMR Lab, Modern Anal & Testing Ctr, Changsha 410013, Hunan, Peoples R China
来源：
DYES AND PIGMENTS | 2014年 / 107卷
基金：
中国国家自然科学基金;
关键词：
Fluorescence probe; Dansyl; Copper ion; Pyridine; Imaging; Living cells; CU(II); SENSOR; COPPER; FLUOROIONOPHORE; CHEMOSENSOR; ZINC;
D O I：
10.1016/j.dyepig.2014.02.022
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A novel fluorescent quenching probe (HMPM-NS) bearing a dansyl and pyridine group has been successfully developed. This probe showed high selectivity and sensitivity for the detection of copper ion over commonly coexistent metal ions or anions in neutral aqueous media. The limit of detection of this probe based on 3 x delta(blank)/k was calculated as low as 0.168 mu M of Cu2+. And the interaction mode with 1:1 stoichiometry was proposed between HMPM-NS and Cu2+. It can be used in almost the entire alkalinity pH window (from pH 4.8-13.5) with a fast response when reacts with Cu2+. On the account of its excellent spectroscopic properties, We demonstrated this probe displays good cell permeability by 3D cell imaging and then successfully applied it to visualize Cu2+ in living cells, which further revealed its valuable applications in biological systems. (C) 2014 Elsevier Ltd. All rights reserved.
引用
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页码：1 / 8
页数：8
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