In vitro activity of chlorogenic acid against Aspergillus fumigatus biofilm and gliotoxin production

被引：4
|
作者：
Kong, Jin-Liang [1 ]
Luo, Jing [1 ]
Li, Bing [1 ]
Dong, Bi-Ying [1 ]
Huang, Hong [1 ]
Wang, Ke [1 ]
Wu, Li-Hong [1 ]
Chen, Yi-Qiang [1 ]
机构：
[1] Guangxi Med Univ, Inst Resp Dis, Affiliated Hosp 1, 6 Shuangyong Rd, Nanning 530021, Guangxi, Peoples R China
来源：
EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2017年 / 13卷 / 06期
基金：
中国国家自然科学基金;
关键词：
chlorogenic acid; Aspergillus fumigatus; biofilm; amphotericin B; gliotoxin; AMPHOTERICIN-B;
D O I：
10.3892/etm.2017.4317
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aspergillus (A.) fumigatus, one of the most common causes of life-threatening fungal infections in immunocompromised patients, shows resistance to antifungal agents as has a high propensity to forming a biofilm. The present study aimed to investigate the effects of chlorogenic acid (CRA) on A. fumigatus biofilm formation and integrity. Confocal laser scanning microscopy was performed to determine the inhibitory effects of CRA against A. fumigatus biofilm formation. Transmission electron microscopy was performed to investigate the ultrastructural changes of A. fumigatus biofilm after CRA exposure. High-performance liquid chromatography and reverse-transcription quantitative PCR were performed to determine the expression of gliotoxin production in biofilm culture. The results showed that CRA at sub-minimum inhibitory concentrations inhibited A. fumigatus biofilm formation. In addition, CRA could decreased the gliotoxin production in the biofilm culture supernatant through inhibiting the expression of master genes involved in gliotoxin biosynthesis. The present study provided useful information for the development of novel strategies to reduce the incidence of A. fumigatus biofilm-associated diseases.
引用
收藏
页码：2637 / 2644
页数：8
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