Simiduia litorea sp nov., isolated from seashore sediments of the Sea of Japan

An aerobic, Gram-stain-negative, agarolytic rod-shaped bacterium, designated KMM 9504(T), was isolated from a sediment sample collected from the seashore of the Sea of Japan. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9504(T) belonged to the genus Simiduia as it was most closely related to Simiduia areninigrae KCTC 23293(T) (97.3 % sequence similarity). Strain KMM 9504(T) was characterized by the major ubiquinone Q-8, and by the predominance of C-16:1 omega 7c, C-17:1 omega 8c, followed by C-16:0, C-15:0, C-17:0 and C-12:1 in its fatty acid profile. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid, an unknown aminolipid, unknown phospholipids, and unknown lipids. Based on the distinctive phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization results, it is concluded that strain KMM 9504(T) represents a novel species of the genus Simiduia, for which the name Simiduia litorea sp. nov. is proposed. The type strain of the species is strain KMM 9504(T) (=NRIC 0917(T)=JCM 19759(T)).