Huoxue Qianyang Qutan Recipe Protects against Early Renal Damage Induced by Obesity-Related Hypertension via the SIRT1/NF-κB/IL-6 Pathway: Integrating Network Pharmacology and Experimental Validation-Based Strategy

被引：2

作者：

Wang, Mingzhu ^{[1
]}

Li, Jianhua ^{[1
]}

Gui, Mingtai ^{[1
]}

Lu, Bo ^{[1
]}

Yao, Lei ^{[1
]}

Zhou, Xunjie ^{[1
]}

Shi, Moyi ^{[1
]}

Hu, Liang ^{[2
]}

Fu, Deyu ^{[1
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Acad Integrat Med, Shanghai, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2022年 / 2022卷

基金：

中国国家自然科学基金;

关键词：

INSULIN-RESISTANCE; PATHOPHYSIOLOGY;

D O I：

10.1155/2022/9599090

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Obesity is recognized as not only a major contributing factor to cardiovascular diseases but also an independent risk factor for end-stage renal disease. Previous studies have found that Huoxue Qianyang Qutan Recipe (HQQR) could reduce urinary microalbumin in patients with obesity-related hypertension (OBH). However, the renal protective activity of HQQR in OBH and its molecular targets involved remains ambiguous. In this work, we investigate the mechanism of HQQR against OBH-induced early renal damage using integrating network pharmacology and experimental validation-based strategy. First, via network pharmacology, IL-6 is identified as one of the key targets of HQQR against early renal damage in hypertension, and inhibition of inflammation is a crucial process. Second, in in vivo experiments, HQQR can lower blood pressure, lose weight, and restore metabolic abnormalities in OBH rats, which could be associated with the effects on protecting early renal damage. Finally, in the mechanism, HQQR increases SIRT1 mRNA and protein expression consistent with reduction of NF-kappa B acetylation and suppressed the p65-mediated inflammatory signaling pathway. As a result, HQQR robustly inhibits OBH-induced renal inflammation by reducing IL-6 mRNA and protein levels in the renal tissue and the release of IL-6 in serum of OBH rats. This study aims to provide a multimethod (network pharmacology-animal experiment) and multilevel (component-target-pathway) strategy for the prevention and treatment of OBH-induced target organ damage by traditional Chinese medicine.

引用

页数：10