Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column

Using vinyl ester resin as monomer, ethylene gylcol dimethacrylate (EDMA) as the crosslinker, dodecanol as porogenic solvents and 2, 2' - azobisisobutyronitrile (AIBN) as initiators, a poly (vinyl ester resin-co-ethylene dimethacrylate) monolithic column was prepared by "in-situ" process. The monolith was further derivatized by 3-glycidoxypropyltrimethoxysilane and then hydrolyzed in 0.25 mol/L sulfuric acid to prepare diol column. The effects of pH and buffer concentration on the retention of IgG were investigated and the separation of IgG from human plasma was realized. the separation of IgG from chicken egg yolk in different buffer concentration and the effect of spacer arm on the separation were also investigated. The results showed that the monolith with spacer arm had a higher separation capacity. Moreover, the adsorption capacity of the column was examined and the maximum adsorption amount was 35 mu g/g.