Cooling-rate screening system for determining protein crystal growth conditions

被引：12

作者：

Murai, Ryota

Nakata, Shinya

Kashii, Masafumi

Adachi, Hiroaki

Niino, Ai

Takano, Kazufumi

Matsumura, Hiroyoshi

Murakami, Satoshi

Inoue, Tsuyoshi

Mori, Yusuke

Sasaki, Takatomo

机构：

[1] Osaka Univ, Dept Elect Elect & Informat Engn, Sasaki Lab, Suita, Osaka 5650871, Japan

[2] Osaka Univ, SOSHO Project, Suita, Osaka 5650871, Japan

[3] JST, CREST, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Dept Orthopaed Surg, Fac Med, Grad Sch Med, Suita, Osaka 5650871, Japan

[5] SOSHO Inc, Osaka 5670085, Japan

[6] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan

[7] JST, PRESTO, Suita, Osaka 5650871, Japan

[8] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan

[9] Osaka Univ, Dept Cell Membrane Biol, Inst Sci & Ind Res, Osaka 5670047, Japan

来源：

JOURNAL OF CRYSTAL GROWTH | 2006年 / 292卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

biocrystallization; nucleation; seed crystals; biological macromolecules; lysozyme; proteins;

D O I：

10.1016/j.jcrysgro.2006.04.050

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The cooling method is of major importance in the making of large crystals of high quality, but there has been no tool to efficiently determine an optimum cooling rate for protein crystal growth. We previously developed a new temperature control tool, temperature-at-once (TAON), which could produce multi-temperatures on a single microplate by generating a temperature gradient. We have now added a new function to control the cooling rate in TAON with a personal computer. The improved TAON enables us to grow protein crystals at multiple cooling rates on a single microplate at a time. We can now search more efficiently for an optimum cooling rate for crystal growth. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：433 / 436

页数：4

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