In this paper, we identified the defect state of p-channel low temperature poly-Si thin film transistors (LTPS TFTs) fabricated on a polyimide (PI) substrate that is distributed differently in the interface and channel areas depending on the activation annealing temperature, as well as observed the effect on the threshold voltage (V-T) and reliability. To verify the effect of the process temperature on the performance of the LTPS TFTs, an activation heat treatment was conducted at 400 degrees C, 370 degrees C and 340 degrees C. More defects were distributed in the interface and channel area of LTPS TFTs with low heat treatment temperature, resulting in V-T formed at a higher voltage. After the negative bias temperature instability (NBTI) test, the V-T shifted in the negative voltage direction and moved -0.13 V, -0.51 V and -0.72 V from LTPS TFTs with high activation annealing temperature. In the hot carrier instability (HCI) test, V-T also shifted to the negative direction of -0.19 V, -0.51 V, and -1.07 V. The defects distributed in the interface and channel areas that are not curated or activated due to the low activation annealing temperature caused instability under the bias stress.