Two isoforms of leptin in the White-clouds Mountain minnow (Tanichthys albonubes): Differential regulation by estrogen despite similar response to fasting

被引：30

作者：

Chen, Ting ^{[2
,3
]}

Chen, Shuang ^{[4
]}

Ren, Chunhua ^{[2
,3
]}

Hu, Chaoqun ^{[2
,3
]}

Tang, Dongsheng ^{[1
]}

Yan, Aifen ^{[1
]}

机构：

[1] Foshan Univ, Coll Med, Foshan, Peoples R China

[2] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China

[3] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China

[4] Univ Hong Kong, Dept Anat, Hong Kong, Hong Kong, Peoples R China

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 2016年 / 225卷

基金：

中国国家自然科学基金;

关键词：

Leptin; Cyprinid fish; Molecular cloning; Fasting; Estrogen; Gene expression; OVERLAPPING TRANSCRIPT GENES; ORANGE-SPOTTED GROUPER; CARP CYPRINUS-CARPIO; FOOD-INTAKE; ATLANTIC SALMON; RECEPTOR EXPRESSION; TISSUE DISTRIBUTION; GENOME DUPLICATION; MOLECULAR-CLONING; PLASMA LEPTIN;

D O I：

10.1016/j.ygcen.2015.08.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Leptin has been well-established as a canonical anorexic peptide hormone in mammals, though much of its function in fish remains obscure. In this study, the cDNAs of two leptin isoforms (leptin-A and leptin-B) were cloned from the liver of a small cyprinid fish, Tanichthys albonubes. The two T. albonubes leptins, sharing low primary amino acid sequence homology with their mammalian counterparts, and between themselves, are highly conserved in three-dimensional protein structures and gene structures. Liver is a major source of leptin mRNA in T. albonubes with leptin-A being the dominant form. The expression of hepatic leptin-A but not leptin-B mRNA in female fish is significantly higher than in male fish. Transcriptional hepatic levels of leptin-A and leptin-B in both male and female fish were demonstrated to increase after long-term fasting (10-25 days) but decline upon re-feeding (3 days). Strikingly, estrogen (E-2) administration induced only leptin-A but not leptin-B hepatic mRNA expression in both male and female fish. Our study here provides the first evidence for differential regulation of two leptins in fish, and sheds new light on the possible origin of leptin in lower vertebrates. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：174 / 184

页数：11