Stimulation of human monocytes with the Gram-positive vaccine vector Streptococcus gordonii

被引：19

作者：

Ciabattini, Annalisa ^{[1
]}

Cuppone, Anna Maria ^{[1
]}

Pulimeno, Rita ^{[1
]}

Iannelli, Francesco ^{[1
]}

Pozzi, Gianni ^{[1
]}

Medaglini, Donata ^{[1
]}

机构：

[1] Univ Siena, Dipartimento Biol Mol, LAMMB, Policlin Le Scotte, I-53100 Siena, Italy

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2006年 / 13卷 / 09期

关键词：

D O I：

10.1128/CVI.00110-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Streptococcus gordonii is a bacterial vaccine vector which has previously been shown to activate dendritic cells in vitro and to induce local and systemic immune responses in vivo. In the present study, human monocytes (THP-1 cell line and peripheral blood monocytes) were characterized following interaction with S. gordonii. Treatment of human monocytes with S. gordonii but not latex beads induced a clear up-regulation of CD83, CD40, CD80, and CD54 and the down-regulation of CD14. Furthermore, bacterial treatment stimulated an increased expression of Toll-like receptor 5 (TLR5), TLR6, and TLR7, production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin 1 beta, and reduction of the phagocytic activity. This work shows that the immunostimulatory activity of S. gordonii is not restricted to induction of dendritic-cell maturation but also affects the differentiation process of human monocytes.

引用

页码：1037 / 1043

页数：7

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