Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals

被引:4
|
作者
Nakamura, Yuki [1 ]
Baba, Seiki [1 ]
Mizuno, Nobuhiro [1 ]
Irie, Takaki [1 ]
Ueno, Go [2 ]
Hirata, Kunio [2 ]
Ito, Sho [3 ]
Hasegawa, Kazuya [1 ]
Yamamoto, Masaki [2 ]
Kumasaka, Takashi [1 ]
机构
[1] Japan Synchrotron Radiat Res Inst, Prot Crystal Anal Div, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan
[2] RIKEN SPring 8 Ctr, Adv Photon Technol Div, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan
[3] Rigaku Corp, Applicat Labs, ROD Single Crystal Anal Grp, 3-9-11 Matsubara Cho, Akishima, Tokyo 1968666, Japan
关键词
protein crystallography; cryocrystallography; removal of frost; liquid-nitrogen drizzling device; automated data collection; STRUCTURAL GENOMICS BEAMLINES; MACROMOLECULAR CRYOCRYSTALLOGRAPHY; PROTEIN CRYSTALS; CRYSTALLOGRAPHY; MOSAICITY; SPRING-8; LIMITS;
D O I
10.1107/S2053230X2001420X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservation provides simple storage of crystals and easy transportation to a synchrotron. However, this technique sometimes results in the undesirable adhesion of frost to mounted crystals. The frost produces noisy diffraction images and reduces the optical visibility of crystals, which is crucial for aligning the crystal position with the incident X-ray position. To resolve these issues, a computer-controlled device has been developed that drizzles liquid nitrogen over a crystal to remove frost. It was confirmed that the device works properly, reduces noise from ice rings in diffraction images and enables the centering of crystals with low visibility owing to frost adhesion.
引用
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页码:616 / 622
页数:7
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