In Vivo Targeting of Hydrogen Peroxide by Activatable Cell-Penetrating Peptides

被引：205
|
作者：
Weinstain, Roy [1 ]
Sayariar, Elamprakash N. [1 ]
Felsen, Csilla N. [1 ]
Tsien, Roger Y. [1 ,2 ,3 ]
机构：
[1] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 03期
关键词：
OXIDATIVE STRESS; D-PENICILLAMINE; NADPH OXIDASE; H2O2; NANOPARTICLES; INFLAMMATION; SUPEROXIDE; MECHANISMS; CHEMISTRY; APOPTOSIS;
D O I：
10.1021/ja411547j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.
引用
收藏
页码：874 / 877
页数：4
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