Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea

被引：81
|
作者：
Chen, Haixia [1 ]
Wang, Zhaoshuai [1 ]
Qu, Zhishuang [1 ]
Fu, Lingling [1 ]
Dong, Peng [1 ]
Zhang, Xin [1 ]
机构：
[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2009年 / 229卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Oolong tea polysaccharide; Composition; Antioxidant activity; Atomic force microscopy; Laser light scattering analysis; CHEMICAL-CHARACTERIZATION; GREEN; PURIFICATION; COMPONENTS; ANTITUMOR; ACID;
D O I：
10.1007/s00217-009-1088-y
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Oolong tea polysaccharide (OTPS) is a kind of water-soluble polysaccharide isolated from oolong tea. Its molecular weight, composition, and structure were analyzed by laser light scattering analysis, chemical methods, gas chromatography, and atomic force microscopy (AFM). Antioxidant potential was detected by hydroxyl radical scavenging, inhibition of lipid peroxidation, DPPH radical scavenging, and reducing power assays. Results showed that OTPS was a non-reductive acid heteropolysaccharide bound to protein. The amounts of uronic acid, protein and neutral sugar in OTPS were 40.65, 19.59, and 26.66%, respectively. The neutral sugar was composed of d-rhamnose, l-arabinose, d-galactose, and d-glucose with a molar ratio of 1.37:1.89:1.00:1.30. The molecular weight of OTPS was 1.28 x 10(6) Da and the second viral coefficient was 2.36 x 10(-4) cm(3) mol/g(2). A globular image of OTPS was imaged by AFM. OTPS showed effective antioxidant activities on hydroxyl radical scavenging assay and inhibition assay of lipid peroxidation.
引用
收藏
页码：629 / 635
页数：7
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