The moon is an ideal on-orbit radiation calibration source. In order to improve the measurement accuracy of long-wave infrared radiation for satellites and other on-orbit aircrafts, the characteristics of lunar long-wave infrared radiation are studied in this paper. The law of lunar long-wave infrared radiation is explored taking the moon as a point target or disk target, and the calibration accuracy is calculated based on the measured space-based data by analyzing the types of lunar radiation sources and radiation detection mechanism, and by establishing a radiation detection model. First, the lunar radiation is studied from two aspects of lunar self radiation and reflected solar radiation. The lunar surface temperature is fitted accurately, the lunar infrared radiation model is established, and the mathematical relationship is simulated between the radiation measurement results of space-based satellites and the lunar phase angles. Second, the key parameters of con-version between gray-scale and irradiation images are obtained through calibrating the radiation of satellite infrared loads in the ground. The lunar long-wave infrared radiation measurement model is then established. Finally, the reflectance and emissivity of the lunar surface are calculated based on the measured data taken by the "Jilin-1" satellite on the moon. The experimental results show that the long-wave infrared radiation char-acteristics of the lunar surface obtained by the proposed menthod are accurate and robust. Moreover, there are nearly 7.18% and 5.71% of the fitting errors in the obtained data compared with Apollo 12 070 laboratory measurement results and diviner measured data, respectively.